Scientists from the Laboratory of Quantum Optics of the Faculty of Physics at the University of Warsaw, in collaboration with colleagues from the Weizmann Institute of Science in Israel, have created a microscope that has no resolution limit. The results have been published in the journal Optica.
The development of biology and medicine requires more detailed structures and relationships of ultra-small objects, such as proteins in cells. An ordinary microscope is not enough for this and only inanimate objects can be examined with electronic microscopes, as the sample must be placed in a vacuum.
The new modification of the microscope works on the basis of the already known scanning image microscope (ISM) method. Thus, the correlation of intensities detected by several detectors is calculated. In this connection, measuring the n-th order correlations increases the resolution by 2n times, so the new method of the microscope has no resolution limit.
According to the scientists, the new method requires low costs, only a confocal microscope is needed, in which it is necessary to replace the photomultiplier tube with a matrix detector.
In this way, a microscope can be created with unprecedented detail, four times the diffraction limit.